2B8 Epitope Tag is the solution of ChIP
The Next-Generation Neutral Epitope Tag for Chromatin Biology (ChIP-seq)
1. Executive Summary
In recombinant protein expression, purification, and protein-protein interaction (PPI) analysis, the choice of an epitope tag is critical for data reliability. Traditional tags like FLAG, HA, and c-Myc exhibit notable limits under specific experimental setups, including charge bias, endogenous cross-reactivity, and chromatin structure distortion.
The 2B8 Epitope Tag is an ultra-compact, near-neutral 9-amino acid sequence (RDPLPFFPP) designed with advanced protein engineering to completely bypass these obstacles while preserving native protein folding.
2. 4 Core Premium Values
• Ultra-Compact Structure: Only 9 amino acids. Minimizes interference with target protein folding, 3D structure, PPI, and biological activity.
• Near-Neutral Charge (Net Charge ~0): Avoids electrostatic charge distortion. Unlike the strongly negative FLAG tag (-5), c-Myc (-2), HA (-2) it perfectly guards charge-sensitive protein environments.
• High Affinity & Specificity: Near-zero cross-reactivity with mammalian endogenous proteins. Completely blocks background bands in Western Blot and IF/FACS.
• Gentle Native Elution: Facilitates competitive elution using 2B8 synthetic peptides under mild, physiological conditions (pH 7.4), leaving fragile multi-protein complexes intact.
3. Performance Comparison Matrix
4. Mechanistic Supremacy in ChIP & ChIP-seq
Chromatin Immunoprecipitation (ChIP) maps genomic binding spots of transcription factors. The 2B8 tag bypasses longstanding functional bottlenecks that sabotage standard tags:
1. Eradication of Electrostatic Repulsion: Traditional FLAG tags carry negative charges (DDD) that clash with the DNA phosphate backbone, altering chromatin architectures. The neutral 2B8 tag enables targets to associate naturally without artifactual repulsion.
2. Uncompromised Epitope Exposure Post-Fixation: During formal cross-linking, the 2B8 peptide sequence remains outward-facing and highly accessible, allowing Anti-2B8 Monoclonal Antibodies to pull down targets with unparalleled yield.
3. Maximal Peak Resolution & S/N Ratio: By completely eliminating false-positive peaks derived from non-specific electrostatic clutter, researchers achieve exceptionally sharp, publication ready Peak Calls across genome-wide maps.
5. Primary Application Fields
• Western Blotting & IF/IHC:
• Guarantees high-specificity single bands without background interference. Ideal for tracking subtle subcellular distribution patterns.
• IP / Co-IP & Native Complex Purification:
• Captures trace multi-protein assemblies gracefully. Enables native elution strategies utilizing competitive free 2B8 peptides.
6. Ordering Information & Product Lineup
7. Contact & Global Support
Manufacturer: BioJane Com. Ltd.
Technical Support: shbhoo@khu.ac.kr
Website: www.biojane.net or www.poptagab.com
USA International Distributor: www.poptagab.com
Website: www.biojane.net | USA Distributor: www.poptagab.com This is for informational purposes only. For medical advice or diagnosis, consult a professional.
