POP(2B8) Tag Antibody Data Sheet

* BACKGROUND

POP (Phytochrome-Originated Peptide) epitope sequence (RDPLPFFPP, CGG GAC CCG TTG CCC TTT TTT CCA CCG) is a part of Deinococcus radiodurans Bacteriophytochrome (DrBphP). This monoclonal anti-POP antibody is highly specific to any protein containing minimal original epitope sequence (RDPLPFFPP).

* SOURCE

POP epitope is amino acid sequence (3-11) of Deinococcus radiodurans bacteriophytochrome (DrBphP, 75kDa). POP(2B8) antibody has been generated by immunizing the purified DrBphP protein into mice.

* APPLICATIONS

POP(2B8) antibody is recommended for detection of RDPLPFFPP or RDPLPAFPP peptide tagged fusion proteins by Western Blot (starting dilution 1:2,500, dilution range 1:2,500~1:20,000), Immunoprecipition [~5 μg per 100-500 μg of total protein (1 ml of cell lysate)], Solid phase ELISA (dilution range 1:1,000-1:10,000), Immunofluorescence (starting dilution 1:500, dilution range 1:500-1:2,000, Chromatine immunoprecipitation(ChIP).

* STORAGE

Store at -20 ℃. Stable up to one year from the date of receipt. Non-hazardous. No MSDS required.

* PRODUCT ( Not intended for clinical use. )

POP(2B8) Ab : Each vial contains 100 μg IgG (monoclonal,HC:IgG2a/LC:k) in 100 μl of PBS with < 0.1% sodium azide, 30 % glycerol.

Included Positive control protein : Purified POP(2B8) tagged GFP protein (~28 kDa, SDS sample buffer added - ready to use). Use 5 μl for Western blot.

* REFERENCES

Recombinant haemostatic protein for therapeutic substitution of platelet function via tripartite
haemostatic mechanisms in thrombocytopenic male mice. Lim CG et. al. Nat. Commun. (2026) 17(1) : 4702.

HDAC6 regulates BACE1 stability and NLRP3 inflammasome activation in Alzheimer's disease. Lee J et. al. Brain. (2026) 00: 1-15.

A Small Epitope Tagging on the C-Terminus of a Target Protein Requires Extra Amino Acids to Enhance the Immune Responses of the Corresponding Antibody. Lee K et. al. J. Microbiology.(2024) 13;34(6):1222-1228.

Antibody production and characterization of the nucleoprotein of sever fever with thrombocytopenia syndrome virus (SFTSV) for effective diagnosis of SFTSV. Lee K et. al. Virology Journal, (2023) 20:206.

HYL1-CLEAVAGESUBTILASE1(HCS1) suppresses miRNA biogenesis in response to light-to-dark
transition. Jung HJ et. al. Proc Natl Acad Sci. USA. (2022) 119(6).

Flashlight into the Function of Unannotated C11orf52 using Affinity Purification Mass Spectrometry. Yang Y et.al. J. Proteome Res. (2021) 20(12):5340-5346.

Light Triggers the miRNA-Biogenetic Inconsistency for De-etiolated Seedling Survivability in Arabidopsis thaliana. Choi SW et.al. Molecular Plant. (2020) 13(3).

Arabidopsis RING E3 ubiquitin ligase JUL1 participates in ABA-mediated microtubule depolymerization, stomatal closure, and tolerance response to drought stress. Yu SG et.al. Plant J. (2020) 103(2):824-842.

POP(2B8) Tag POP Tag Antibody Ab Epitope Protein

Specificity test of POP(2B8) Ab to total proteins of various organisms.

Coomassie blue staining(left) and Western blotting(right) analysis against various model organisms. 1 : Rice; 2 : Nicotiana benthamiana; 3 : Arabidopsis thaliana; 4 : Saccharomyces cerevisiae; 5 : E.coli; 6 : Dorosophila melanogaster S2; 7 : Pre–B cell PD36; 8 : Purified DrBphP(control). POP(2B8) Ab has no non-specific immune response to total proteins of tested organisms.

Immunofluorescence image showing mosaic ubb:POP(2B8)-EGFP expression in the trunk of a 4-dpf zebrafish larva (Courtesy of Dr. Shin, Univ. of Pittsburgh).

Specificity test of POP(2B8) Ab to total proteins of various human cell lines.

1 : HEK-293(Human kidney) 2 : HepG2(Human liver) 3 : C33A(Human cervix) 4 : CRL-2302(Human eye) 5 : Human-hepatocyte 6 : Mouse-hepatocyte 7 : POP(2B8)-Sox9 expressed HEK-293 lysate

POP(2B8) Ab has no non-specific immune response to total proteins of tested human cell lines.